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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Anal Biochem. 2009 Oct 7;397(1):73–78. doi: 10.1016/j.ab.2009.09.048

Figure 1. A cartoon representation of the peptide-based biosensor for the c-Abl kinase.

Figure 1

The substrate contains a biotinylated “reporter” module that is phosphorylated by c-Abl; a “targeting” module binds the Abl SH3 domain; and a “transduction” module (the TAT peptide), to aid the peptide in crossing the membrane barrier. The reporter and targeting sequences are connected via a photocleavable linker to allow release of the reporter from the backbone of the biosensor.