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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Anal Biochem. 2009 Oct 7;397(1):73–78. doi: 10.1016/j.ab.2009.09.048

Figure 2. Cellular uptake and distribution of substrate peptide.

Figure 2

HEK293 cells were treated with Alexafluor-555-labeled Abl biosensor peptide (red, 25 μM) for 15 min (left panel) or 4 h (right panel) at 37 °C in media. Nuclei in the live cells were stained with Hoechst 33342 (blue). Cells were washed with PBS and visualized using a Zeiss Axiovert fluorescence microscope within 5 min. Peptide was rapidly taken up and distributed throughout the cell (15 min), persisting up to 4 h.