Table 1.
Pyrosequencing information.
| Sequencing primer |
|||
|---|---|---|---|
| BPHD-f3 | BPHD-r1 | Sum | |
| Number of raw sequences | 2486 | 835 | 3321 |
| Total raw sequence length | 556 744 bp | 198 152 bp | 754 896 bp |
| Average raw sequence length | 224 bp | 237 bp | |
| Obtained sequencea length | 175 bp (58 aa) | 200 bp (66 aa) | |
| Number of obtained sequences | 2024 | 608 | 2632 |
| Number of valid sequencesb | 2000 | 604 | |
| Unique nucleotide sequences in valid sequences | 743 | 339 | |
a
Sequences that passed position of error and frameshift analysis
b
The number of sequences which have 230D, 233H and 239H for BPHD-f3 alignment and 344P for BPHD-r1 alignment. (The number correspond to the position of bphA1 from B. xenovorans LB400.