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Kinetic parameters for mutants of Sco3058a
| Enzyme | kcat (s−1) | Km (mM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| WT | 132 ± 2 | 1.8 ± 0.1 | (7.2 ± 0.4 ) × 104 |
| D22H | <0.03 | ||
| H150N | 0.7 ± 0.01 | 4.9 ± 2.1 | (1.4 ± 0.4) × 102 |
| H150A | 7.2 ± 0.4 | 4.3 ± 0.5 | (1.7 ± 0.2) × 103 |
| R223K | 0.4 ± 0.04 | 8.8 ± 1.4 | 46 ± 9 |
| R223M | <0.03 | ||
| D320N | <0.03 | ||
| D320A | <0.03 |
These constants were obtained by a fit of the data to equation 2.
