Table 4.

Oligonucleotides Used in the Construction of PAX3-NCOA1 and PAX3-NCOA2 Deletion Mutants

Gene	Primer	Sequence
PAX3- NCOA1 type 1	AD1-PN1.1-357u	GCTATAAATCAGAGTAAATCAGCTCTTGAACAGCTGGTATCC′
	AD1-PN1.1-357l	GGATACCAGCTGTTCAAGAGCTGATTTACTCTGATTTATAGC
	AD1-PN1.1-417u	GCTATAAATCAGAGTAAATCGGTGGATTAGATGTATTATCAGAG
	AD1-PN1.1-417l	CTCTGATAATACATCTAATCCACCGATTTACTCTGATTTATAGC
	AD2-PN1.1-697-727u	GTTCCCCAAGGTGAGGCCAACGCCTCCGGGTATCAGACATGA
	AD2-PN1.1-697-727l	TCATGTCTGATACCCGGAGGCGTTGGCCTCACCTTGGGGAAC
PAX3- NCOA1 type 2	AD1-PN1.2-495u	GAAGACCAGTGTATTAGCGAGAAGGCTTCTTCTTGA
	AD1-PN1.2-495l	TCAAGAAGAAGCCTTCTCGCTAATACACTGGTCTTC
	AD1-PN1.2-549u	GAAGACCAGTGTATTAGCGGTGGATTAGATGTATTAATCAG
	AD1-PN1.2-549l	CTGATTAATACATCTAATCCACCGCTAATACACTGGTCTTC
	AD2-PN1.2-835-865u	GGTGAGGCCAACTTTGCTCCATCTCTAGACATGAAGGCCTGGCAGCAA
	AD2-PN1.2-835-865l	TTGCTGCCAGGCCTTCATGTCTAGAGATGGAGCAAAGTTGGCCTCACC
PAX3- NCOA2	AD1-PN2-649u	GCCAGCCAAAACAGGCAGCCAGACCAGGTGTATCTGGCCTTGCGG
	AD1-PN2-649l	CCGCAAGGCCAGATACACCTGGTCTGGCTGCCTGTTTTGGCTGGC
	AD1-PN2-739u	GCCAGCCAAAACAGGCAGCCAGACCAGGTAGCTATTCGCCCATGCAAGATCCA
	AD1-PN2-739l	TGGATCTTGCATGGGCGAATAGCTACCTGGTCTGGCTGCCTGTTTTGGCTGGC
	AD2- PN2-898-93u	CAGCAGTTTCCATTTCCTCCAAACTACCCAACAGTCTCAGGCCAACCCAGCC
	AD2- PN2-898-931	GGCTGGGTTGGCCTGAGACTGTTGGGTAGTTTGGAGGAAATGGAAACTGCTG

u= upper strand primer; l=lower strand primer