Figure 6.

Nuclear localization of activated STAT2 is required for ISGF3-mediated gene expression and induction of type I IFN-induced apoptosis. STAT2 deficient H123 cells reconstituted with various forms of STAT2 were left either untreated or treated with IFN-α (3,000 U/ml) for the indicated times. A) Subcellular distribution of STAT2 was analyzed by confocal immunofluorescence microscopy. B) Gene activation of ISGs was assessed by quantitative RT-PCR. Figure shown is representative of three experiments with similar results. C) Apoptosis was measured by dually staining of cells with Annexin V and PI. Standard error bars were determined from three independent experiments.