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Effects of recombinant osteopontin (OPN) treatment on matrix metalloproteinase (MMP)-9 induction and tissue inhibitor of MMP (TIMP)-1 reduction in the left cerebral hemisphere at 24 hours after subarachnoid hemorrhage (SAH). Western blots for MMP-9 (A), TIMP-1 (B), laminin (C) and zona occludens (ZO)-1 (D). Expression levels of each protein in Western blot are expressed as a ratio of β-actin levels for normalization. N=6 rats per group; error bar, SD.
