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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Anal Biochem. 2009 Oct 13;397(1):107–114. doi: 10.1016/j.ab.2009.10.017

Figure 3.

Figure 3

(a) Change in the retention factor for R-propranolol as function of mobile phase volume for pH 7.4, 0.067 M potassium phosphate buffer as it was passed through a 1 cm × 2.1 mm i.d. HDL column at 1 ml/min and 37°C. (b) Typical frontal analysis results obtained for the application of R-propranolol to a 5.0 cm × 2.1 mm i.d. HDL column at analyte concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, 1, 2, 5, 10 or 25 μM; these results were also obtained at 1.0 ml/min and 37°C in the presence of pH 7.4, 0.067 M potassium phosphate buffer.