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Observation of TMR adhering to a glass cover slide in a flow cell setup with ascorbase in solution. Titration of 25.5 mM L-ascorbic acid removes the oxygen in controlled discrete steps. (A) Dissolved oxygen as measured by a commercial probe (Mettler-Toledo). (B) Triplet lifetime measurement using an average intersystem crossing of τisc = 0.69 μs obtained by a global fitting over seven measurements. (C) Triplet-state relaxation rate.
