Table 3.
Pretreatment time (min) | 2 | 5 | 7 | 10 |
---|---|---|---|---|
FJC | *++++ | +++ | +++ | ++ |
IF | ++++ | +++ | ++ | – |
Note: Intensity of FJC or IF (NeuN): ++++, highest intensity; +++, high intensity; ++, moderate intensity; –; background intensity.
*The intensity of the FJC background was so high that the sample showed a low stain-to-noise ratio.