Fig. 4.

Comparison of immunohistochemical distribution of 13(R)-HODE (A, C, E, G) with OxPC (B, D), PPARγ (F) and CD36 (H) in lesional cells of human atherosclerotic carotid arteries. Areas of panels (A, C, E, G) are the same as those of panels (B, D, F, H) on consecutive sections, respectively. Solid and blank arrowheads and arrows indicate VECs, macrophages and migrating VSMCs, respectively. PIC method using DAB (A–H). Bar=100 µm. Abbreviations: DAB, 3,3'-diaminobenzidine tetrahydrochloride; HODE, hydroxyoctadecadienoic acid; OxPC, oxidized phosphatidylcholine; PIC, polymer-immunocomplex; PPARγ, peroxisome proliferator-activated receptor-gamma; VECs, vascular endothelial cells; VSMCs, vascular smooth muscle cells.