Fig. 1.
De novo generation of PrPSc in Syrian hamsters. a Western blotting of rPrP fibrils annealed in the presence of NBH or BSA. NBH-annealed fibrils showed a PK-resistant product of 16 kDa. Undigested samples were loaded at 1/20th the amount of the digested samples. b Western blotting of BHs from animals inoculated with NBH-annealed fibrils. Animal #36798 showed PK-resistant PrPSc with a characteristic band shift. c Western blotting of BHs from animals inoculated with BH from animal #36798. Without PK treatment, 1/10th of a sample was loaded. d Western blotting of BHs from animals inoculated with 10% BH from animal #36793. Undigested sample was loaded at 1/10th the amount of the digested samples. e Western blotting of BHs from animals inoculated with NBH-annealed fibrils. BHs were subjected to a single round of PMCA that consisted of 40-s sonication pulses applied every hour during a 48-h period, where each sample was its own substrate. Samples in a, b, e were treated with 50 μg/ml PK, and samples in c and d with 20 μg/ml PK. 3F4 antibody was used for a, c–e and R1 antibody was used for b