Table 1.
Bioassay of rPrP amyloid fibrils in golden Syrian hamsters
| Inoculum | n s/n t a | n PK-res/n t b | Clinical onset (days PI) | Euthanized at days PI |
|---|---|---|---|---|
| NBH-annealed rPrP fibrils | 0/6 | 3d/6 | – | 661 |
| BSA-annealed rPrP fibrils | 0/7 | 2e/7 | – | 661 |
| None (age-matched controls) | 0/9 | 0/9 | – | 661f |
| Mock-annealed NBH | 0/7 | 0/7 | – | 660 |
| α-rPrP-monomer mock-annealed with NBH | 0/8 | 0/8 | – | 660 |
| rPrP-fibrilsc | 0/7 | 0/7 | – | 690, 704, 5 at 732 |
| 2nd Passage: 10% BH from age-matched control #1 | 0/5 | 0/5 | – | 661 |
| 2nd Passage: 10% BH from age-matched control #2 | 0/5 | 0/5 | – | 661 |
| 2nd Passage of NBH-annealed fibrils (from #36798) | 8/8 | 8/8 | 481 ± 4 | 523, 561, 561, 568, 574, 586, 586, 601 |
| 2nd Passage of NBH-annealed fibrils (from #36793) | 7/7 | 7/7 | 565 ± 14 | 1 at 655, 6 at 661 |
| 2nd Passage of BSA-annealed fibrils (LMW PK-resistant, from #36787) | 0/7 | 6/7 | – | 661 |
| 2nd Passage of BSA-annealed fibrils (PK-sensitive, from # 36788) | 0/7 | 0/7 | – | 661 |
aNumber of animals with clinical signs over the total number of animals survived to the end of the experiment
bNumber of animals with PK-resistant PrP in BHs over the total number of animals survived to the end of the experiment
cThe rPrP fibrils (S-type) were produced as previously described [22]
dIn one BH PK-resistant PrPSc was found by PK digestion assay, and in two additional BHs PK-resistant PrPSc appeared after a single round of PMCA
eOne BH showed PK-resistant PrP with atypical low molecular weight bands; this and one additional BH showed PK-resistant PrPSc after a single round of PMCA
f Age-matched control animals were not inoculated, but euthanized at the age matching the age of corresponding inoculated groups