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. 2009 Oct 19;587(Pt 24):5819–5830. doi: 10.1113/jphysiol.2009.180372

Figure 3. Select serine residues in the C-terminus of TREK-1 and TREK-2 are required for AMPK-modulation.

Figure 3

A, representative conductance trace shows that Ala substitution at Ser-333 (S333A) blocks AICAR-mediated inhibition of TREK-1. Inset, I–V plot of the S333A mutant TREK-1 current during control (a) and exposure to AICAR (b). Averaged data (n= 6) shows that 2 min exposure to AICAR had no effect on S333A mutant TREK-1 conductance. B, conductance trace shows that Ala substitution at Ser-300 (S300A) also blocked AICAR-mediated inhibition of TREK-1. Inset, I–V plot of the S300A mutant TREK-1 current during control (a) and exposure to AICAR (b). Averaged data (n= 9) show that 2 min exposure to AICAR had no effect on S300A mutant TREK-1 conductance. C, representative conductance trace show that Ala substitutions at Ser-326 and Ser-359 (S326A/S359A) of TREK-2 channels blocks AICAR-mediated inhibition of TREK-2. Inset, I–V plot of the S326A/S359A mutant TREK-2 current during control (a) and exposure to AICAR (b). Averaged data (n= 5) show that 3 min exposure to AICAR had no effect on S326A/S359A mutant TREK-1 conductance.