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. 2009 Oct 19;587(Pt 24):5819–5830. doi: 10.1113/jphysiol.2009.180372

Figure 8. Hypoxia inhibits TREK channels by an AMPK-dependent mechanism.

Figure 8

A, trace of wild-type TREK-1 conductance and bath temperature show that heating from 24°C to 30°C increases TREK-1 activity. At 30°C exposure to hypoxia (measured bath Inline graphic= 11 ± 1 mmHg) for 6 min inhibited channel conductance. Inset, I–V plots of TREK-1 current obtained at 30°C under control conditions (a) and during exposure to hypoxia (b). B, trace of wild-type TREK-1 conductance in the presence of dnAMPK and bath temperature show that heating from 24°C to 30°C increases TREK-1 activity. At 30°C exposure to hypoxia (Inline graphic= 11 ± 1 mmHg) for 6 min had no effect on channel conductance. Inset, I–V plots of TREK-1 current in the presence of dnAMP obtained at 30°C under control conditions (a) and during exposure to hypoxia (b). C, bar graph of average data (n= 4) shows the percentage inhibition of TREK-1 by hypoxia alone and in the presence of dnAMPK.