Figure 6. All sEPSPs and epileptiform bursts were completely abolished by the AMPA/kainate-receptor antagonist, DNQX.

A, frequent sEPSPs as large as 10 mV (Aa, left panel) were present and prolonged epileptiform bursts (Ab, left panel) were consistently elicited in an immature CA3 minislice bathed with a mixture of gabazine (10 μm), SCH50911 (10 μm) and AP-5 (50 μm). DNQX (50 μm) completely abolished the sEPSPs and prolonged population bursts (Aa and Ab, right panels), confirming that the prolonged bursts under these conditions (i.e. prior blockade of GABA_A, GABA_B and NMDA receptors) were solely mediated by AMPA/kainate receptors. B, DNQX similarly eliminated the brief bursts evoked in mature CA3 minislices. Arrows indicate stimulations. Dotted lines in A and B indicate baseline potentials.