Figure 3. Localization of Fto and Ftm transcripts in adult hypothalamus, pancreas and 13.5 dpc embryo.

Fto and Ftm expression by in situ hybridization in (A) medial coronal hypothalamic and (B) sagittal pancreatic sections from 4-week old C57BL/6J males. Positive alkaline phosphatase staining of Fto and Ftm transcript is seen as a dark perinuclear ring. Fto is expressed in the arcuate nucleus (ARC), the dorsal medial hypothalamus (DMH), and the ventral medial hypothalamus (VMH). Ftm expression is restricted to the ARC in the hypothalamus and is expressed at a lower level than Fto. (C) Fto and Ftm in situ hybridization in medial sagittal sections of 13.5 dpc wild type embryos. Positive alkaline phosphatase staining of Fto and Ftm is seen as a dark perinuclear ring. Fto expression is present in the whole embryo, particularly in the brain and spinal cord. In the brain, Fto expression is enriched in the arcuate nucleus (ARC) and mamillary area. Ftm is expressed mainly in the brain and appears restricted to the ARC and mammilary area. (D) Fto and Ftm expression measured by Real Time PCR in whole brains of 13.5 dpc Lep^ob and +/+ mice. Transcript levels were normalized to Gapdh.