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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Am J Physiol Regul Integr Comp Physiol. 2008 Feb 6;294(4):R1185–R1196. doi: 10.1152/ajpregu.00839.2007

Figure 8. CUTL1 regulates the transcription of FTO and FTM.

Figure 8

(A) rs17817449 and rs8050136 are located within CUTL1 recognition sequences as identified by MatInspector (Genomatix; http://www.genomatix.de). Complementary mouse sequence is aligned to the human sequence. Bases that fit the consensus are highlighted. (B) Lightcycler chromatogram showing the crossing point of PCR reactions containing DNA fragments isolated by ChIP with CUTL1, HES1 and mouse IgG antibodies. (C) Pyrosequencing using DNA extracts isolated by ChIP with the Cutl1 antibody. Genomic DNA isolated from fibroblasts heterozygous for rs8050136 (A/C) was used as control. (D) Expression analysis of CUTL1, FTO and FTM in human fibroblasts heterozygous for rs8050136 (A/C) where CUTL1 has been knocked down with siRNA.