Fig. 2.

Morphological change of β-glucan induced cell death in human colon cancer cells SNU-C4. Cells were cultured without resveratrol (A) or with 10, 50, and 100 µg/mL of β-glucan (B, C, and D). Morphology (top): phase-contrast microscopy showed cell shrinkage, irregularity of shape in β-glucan treated cultures. TUNEL assay (bottom): SNU-668 cells stained using TUNEL method. Condensed and marginated chromatin showed to be stained dark brown. The experiments were done triplicate. Scale bar, 100 µm.