Figure 3.

Reactivity of the apoE–Aβ aggregate with different antibodies to Aβ. (a) The ≈40-kDa aggregate is detected with the antibody αAβ42 (specific for residue 42 of the Aβ sequence) after immunoprecipitation with the apoE antibody AB947. As expected, the Aβ antibody does not recognize human plasma apoE. (b) The ≈40-kDa band is also detected after immunoprecipitation with the monoclonal antibody 4G8 (specific for residues 17–21 of Aβ) and immunodetection with the antibody MAB1062 to apoE. This band comigrates with the aggregate observed after immunoprecipitation with the AB947 antibody to apoE.