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. 1998 Dec 22;95(26):15598–15602. doi: 10.1073/pnas.95.26.15598

Figure 4.

Figure 4

Release of Aβ from the aggregate with apoE in AD brains. (a) Immunoprecipitation with the apoE antibody AB947 and immunodetection with the monoclonal anti-apoE antibody MAB1062 show the 34- to 40-kDa band as in Fig. 2a. This band is underrepresented in AD subjects and in the subject homozygous ɛ4 is visible only after a longer exposure. The polyclonal antibody AB947 alone (B) does not detect any band in the region of the apoE–Aβ complex. The lower portion of the gel, immunostained with the monoclonal anti-Aβ antibody 6E10, reveals the typical Aβ pattern for the AD cases, indicating that a portion of Aβ peptides is released from the complex with apoE after SDS/PAGE, only in AD samples. (b) Quantification of the 34- to 40-kDa band detected in immunoblotting after immunoprecipitation with the antibody to apoE AB947 demonstrates a 40% lower reactivity in AD than in control (CO) subjects. ∗, P < 0.005.