Table 1.
Experimental procedures accepted to confirm DNA-binding property of candidate proteins in FlyTF, and GO terms assigned on their basis (as IDA)
| Experimental procedure (literature) | Search term (FlyTF) | GO term |
|---|---|---|
| • Electro mobility shift assay (EMSA) | Retardation assay | GO:0003677 or GO:0043565 |
| • Band shift assay | ||
| • Gel retardation | ||
| • Low ionic strength PAGE | ||
| • Sucrose gradient sedimentation | ||
| SELEX | SELEX | GO:0043565 |
| Affinity chromatography | Affinity chromatography | GO:0003677 or GO:0043565 |
| Yeast 1 hybrid screen | Y1H | GO:0043565 |
| Yeast double interaction screen | Yeast double interaction screen | GO:0043565 |
| • MNase digestion | Footprinting assay | GO:0043565 |
| • DNase I footprint | ||
| • Hydroxyl radical footprint | ||
| Chromatin immunoprecipitation assay | ChIP | GO:0003682 |
| Staining of polytene chromosomes | Staining of polytene chromosomes | not assigned |
For a key to GO terminology, please refer to Supplementary Table S1.