Figure 3.

Comparative analysis and refinement of gene models. Comparative alignments of orthologous proteins from 10 Aspergillus genomes, shown in the Sybil viewer. (A) Structural rearrangement among Aspergillus genomes (here A. nidulans and A. niger CBS 513.88 are emphasized). (B) Presence or absence of introns and different numbers of exons. (C) Gene duplications (paralogs) in one species relative to other species. (D) Intron structure, such as inordinately long introns relative to orthologs in other species. (E) Gene deletion (or creation) in syntenic blocks among species. Differences in structural annotation among species that can be illuminated with the Sybil view, thus leading to refinement of gene models, include missed gene calls, gene truncations, failure to predict small exons and incorrect intron predictions leading to spurious exons or gene merges.