Fig 1.

TLR4 and TLR6 cooperatively mediate the macrophage inflammatory response to oxLDL.

OxLDL-induced chemokine gene expression in (a) wild-type, Tlr2^−/−, Tlr4^−/−, Tlr6^−/− and (b) Myd88^−/− and Trif^−/− macrophages. Gene expression was analyzed by QRT-PCR and the fold increase in mRNA levels in oxLDL stimulated cells (50 μg/ml, 12h) compared to unstimulated cells is reported (*p<0.05, compared to wild type). (c) Expression of IL-1β mRNA in wild type, Tlr4^−/−, Tlr6^−/− and Myd88^−/− macrophages stimulated with oxLDL (50 μg/ml, 12h). (d) OxLDL-induced expression of an NF-κB luciferase reporter gene in HEK293 cells expressing the indicated TLRs. (e) Effect of sMD-2 on oxLDL-induced NF-κB luciferase reporter gene expression. All data are mean ± s.d. of triplicate samples and are representative of an experimental n of 3-4.