Figure 4.

Alignment of the amino acid sequences of the Bm86 homologues from ticks for which Bm86 vaccine efficiency has been documented: Bm86 AUS (Australian strain) (AAA30098), Bm86 MOZ (Mozambique strain) (FJ809946), Ba86 (ABY58969), Bd86 (ABG21131), Haa86 (AAL36024), Ra86-1 (FJ809944) and Ra86-2 (FJ809945). Cross reactive linear B-cell epitopes mapped using pin-coupled peptides by Odongo et al. [5] are boxed, the regions identified using biotin-coupled peptides by the same authors are underlined. Three synthetic peptides used by Patarroyo et al. [33] which induced an immune response against R. microplus are double underlined and EGF-like domains fitting the pattern Cys-Xaa₄₈-Cys-Xaa_3-6-Cys-Xaa_8-11-Cys-Xaa_0-1-Cys-Xaa_5-15-Cys (where Xaa is any amino acid except for cysteine) with 5 or 6 cysteine residues are shaded grey. The phenylalanine at position 507 of Bm86 AUS was predicted to be a cysteine when the sequence of a second cDNA clone from a separate library was determined by Rand et al. [12].