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Immunodetection of Ra86 by Western Blot analysis using ovine Bm86 antiserum. Lanes 1 and 2: R. microplus and R. appendiculatus midgut proteins probed with control serum from a sheep vaccinated with adjuvant only, lanes 3 and 4: R. microplus and R. appendiculatus midgut proteins probed with ovine Bm86 antisera. The arrow on the right indicates the Bm86 and Ra86 proteins.
