Table 3.
Candidate reference genes ranked according to their expression stability as calculated by the Normfinder and geNorm programs.
| R. microplus | R. appendiculatus | R. microplus and R. appendiculatus | |||
|---|---|---|---|---|---|
| geNorm | Normfinder | geNorm | Normfinder | geNorm | Normfinder |
| ELF1A and RPL4 (0.301) | TBP (0.400) | ELF1A and RPL4 (0.371) | GAPDH (0.359) | ELF1A and RPL4 (0.376) | ELF1A (0.477) |
| ELF1A (0.459) | ELF1A (0.424) | GAPDH (0.521) | |||
| H3F3A (0.559) | RPL4 (0.463) | TBP (0.614) | RPL4 (0.481) | TBP (0.619) | TBP (0.549) |
| TBP (0.656) | BTUB (0.485) | GAPDH (0.764) | PPIA (0.580) | H3F3A (0.778) | PPIA (0.555) |
| BTUB (0.771) | PPIA (0.512) | H3F3A (0.856) | TBP (0.583) | PPIA (0.926) | RPL4 (0.563) |
| PPIA (0.824) | H3F3A (0.577) | PPIA (0.959) | H3F3A (0.709) | GAPDH (0.995) | H3F3A (0.623) |
| GAPDH (0.940) | GAPDH (0.649) | ACTB (1.097) | ACTB (0.720) | BTUB (1.074) | BTUB (0.680) |
| ACTB (1.159) | ACTB (0.952) | BTUB (1.203) | BTUB (0.852) | ACTB (1.259) | ACTB (0.962) |
| GST (1.512) | GST (1.450) | GST (1.818) | GST (2.128) | GST (1.795) | GST (1.899) |
The candidates are listed with decreasing expression stability from top to bottom. Average expression stability values are shown between parentheses.