Figure 3. Effect of AEA on cell proliferation versus cell viability in anti-CD3/anti-CD28-stimulated T-lymphocytes.

Purified resting or CD3/CD28-activated T-lymphocytes (1×10⁵ cells/well) were either left untreated or treated with 2.5 µM AEA and/or cannabinoid receptors agonists or antagonists (each at 1.0 µM). Cells were labelled by means of CFDA-staining as well as by 7-AAD dye, and proliferating cells were analyzed with Flow Cytometry as described in Materials and Methods.