Figure 5. Effect of AEA on TNF-α versus IFN-γ production in activated T-lymphocytes.

T-lymphocytes (1×10⁵ cells/well) were either left untreated or treated with 2.5 µM AEA and/or cannabinoid receptors agonists or antagonists (each at 1.0 µM). Cells were then stimulated with PMA/ionomycin for 6 hours and stained intracellularly with anti-TNFα-APC and anti-IFNγ-PE. Levels of intracellular cytokine production were analyzed by flow cytometry, as detailed in Materials and Methods, and represent 8 independent experiments.