Figure 2. Effect of Indo on FRET efficiency in DPPC/Chol (30 mole%).
Effect of Indo on FRET efficiency between t-PnA and DPPE-NBD (A); and between t-PnA and DOPE-NBD (B) in DPPC/Chol (30%). The energy transfer between the t-PnA and DOPE-NBD was significantly decreased (p<0.05), indicating effective phase separation between gel domains and Lo domains. The FRET efficiency between t-PnA and DPPE-NBD was not changed significantly (p>0.05) at all Indo concentrations tested.