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. 2010 Jan 21;5(1):e8825. doi: 10.1371/journal.pone.0008825

Figure 5. Effect of compounds 106, 109 and 136 in unstimulated KIKI splenocytes.

Figure 5

Splenocytes were collected from pooled KIKI spleens (n = 3) or WT spleens. Spleen mononuclear cells were cultured in triplicate for 48 hours with one of HDACi 106, 109 and 136 at 10 µM or with DMSO for KIKI samples. WT samples were cultured in triplicate for 48 hours with. Quantitative real-time RT-PCR was used to determine relative Fxn levels for each condition using RER1 and β2M as control housekeeping genes. DMSO KIKI condition was set to value 1. Error is represented as the standard deviation of the mean from 3 determinations. p<0.001 for 106 and 136 KIKI responses.