Table 3. Oligonucleotide primers used for drDDC amplification and site-directed mutagenesis.

		Primer Sequences
WT	Fw	5′-AAAACATATGGAGGCGCCGGAGTTCAA-3′
	Rv	5′-AAAAGAATTCACTGCTCCTGTTCCATCTCG-3′
T82A	Fw	5′-AAGTTTCATGCCTACTTCCCCGCAGCCAACTCGTATCCAGCGATC-3′
	Rv	5′-GATCGCTGGATACGAGTTGGCTGCGGGGAAGTAGGCATGAAACTT-3′
T82S	Fw	5′-AAGTTTCATGCCTACTTCCCCAGTGCCAACTCGTATCCAGCGATC-3′
	Rv	5′-GATCGCTGGATACGAGTTGGCACTGGGGAAGTAGGCATGAAACTT-3′
H192W	Fw	5′-TACTGCTCGGACCAGGCTTGGTCATCCGTGGAGCGGGCTGGTCTT-3′
	Rv	5′-AAGACCAGCCCGCTCCACGGATGACCAAGCCTGGTCCGAGCAGTA-3′