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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Neurochem. 2009 Oct 27;112(2):410–419. doi: 10.1111/j.1471-4159.2009.06456.x

Fig. 3. p60FE65 is able to translocate to the nucleus.

Fig. 3

A–B: COS cells were transfected with pcDNA3.1-p97FE65 (A) or pcDNA3.1-KOFE65(M327L) (B) and stained with antibody FE518 (red). Nuclei were visualized with DAPI (blue). C: Relative levels of nuclear p97FE65 (A) and p60FE65 (B) were determined by fluorescence quantitation of stained nuclei in p97FE65-positive cells (n =388), and p60FE65 [KOFE65(M327L)]-positive cells (n=382), respectively. Data were presented as mean ± SE, and analyzed by two-tailed t-test. The results showed that there was no significant difference in FE65 levels in the nuclei of the two groups of cells. Size bar equals 10 microns.