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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Neurochem. 2009 Nov 16;112(3):733–743. doi: 10.1111/j.1471-4159.2009.06494.x

Figure 1. Microglial uptake of apoptotic neurons and neuronal blebs.

Figure 1

CFSE-labeled apoptotic neurons (A–D) and neuronal blebs (A,B,E) were added to rat microglia at a ratio of 3:1 (neurons) or 10:1 (neuronal blebs) without serum (B.) or with normal human serum (NHS), normal rat serum (NRS) (C), C1q-depleted human serum (C1qD) or C1qD in which C1q had been reconstituted to 2× or 10× serum concentration (D,E), as indicated, at a final volume of 10%. After incubation for 1 hour at 37°C, microglia were stained with anti CD11b and ingestion of CSFE targets was assessed by flow cytometry with representative dot blots from a single experiment shown in (A.). % uptake was assessed by calculation of the number of CD11b+CFSE+ cells as a % of total CD11b+ cells, with data from a single representative experiment in the absence of serum given in (B.). Average fold enhancement of uptake by sera are shown in (C. – E.) Data are expressed as the average fold enhancement of uptake over no serum control +/- SD. * p<0.05, ANOVA. n>5 (neurons), n=5 (blebs)