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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Neurochem. 2009 Nov 16;112(3):733–743. doi: 10.1111/j.1471-4159.2009.06494.x

Figure 4. Modulation of LPS-induced proinflammatory cytokines released by rat microglia by C1q.

Figure 4

Cytokine levels were measured by Luminex multiplex analysis of the supernatant of unstimulated microglia plated on LabTek chamber slides coated with 8 μg/mL C1q or HSA control, or identically treated cells stimulated with 150ng/mL LPS for 18 hours. (A.) Data shown are average cytokine levels from an individual experiment, measured in triplicate +/- SD. *p<0.05, **p<0.01, students t-test. Dashed line represents the lower limit threshold of detection. ND – not detected. (B.) Results are expressed as fold difference in expression in supernatants of microglia on C1q-coated slides and stimulated with LPS compared to control levels from cells incubated in wells coated with HSA and stimulated with LPS. Data are the average values of 3 separate experiments, each performed in triplicate +/- SD. * p<0.05, ANOVA.