Table 3.
Total expression of mutated receptors.
| Mutant | N | Total expression (% of WT) | Mutant | N | Total expression (% of WT) |
|---|---|---|---|---|---|
| E23A | 9 | 91.4 ± 3.4 | I41A | 9 | 100.1 ± 6.7 |
| E25A | 9 | 88.7 ± 1.7 | T43A | 15 | 116.0 ± 9.4 |
| E47A | 9 | 99.5 ± 2.0 | A44L | 9 | 81.7 ± 6.5* |
| D30A | 12 | 141.5 ± 11.0* | Q45A | 14 | 109.1 ± 13.8 |
| I32A | 9 | 138.1 ± 7.6*** | Y46A | 15 | 196.6 ± 30.0*** |
| Q33A | 9 | 135.0 ± 11.9 | C48A | 9 | 91.5 ± 3.08 |
| G35A | 12 | 126.7 ± 5.3** | Y49A | 9 | 77.6 ± 3.8* |
| T37A | 9 | 112.9 ± 9.3 | I52A | 9 | 176.1 ± 13.3* |
| R38A | 12 | 158.8 ± 7.6*** | P56A | 9 | 111.6 ± 14.9 |
| N39A | 12 | 130.6 ± 7.1** |
Total cellular expression of mutants that showed a significant change in cell surface expression were measured by a whole-cell ELISA. Values are expressed as % WT expression and are means ± s.e.m. **p > 0.05 and 0.01 compared to WT, Mann–Whitney test.