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. 2009 Sep 30;82(2):413–421. doi: 10.1095/biolreprod.109.077891

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© 2010 by the Society for the Study of Reproduction, Inc.

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FIG. 4. — Zonadhesin localization in spermatozoa from three Equus species. Zonadhesin was detected by immunofluorescence on methanol-fixed spermatozoa using antisera to pig zonadhesin holoprotein. Phase contrast (A) and paired epifluorescence (B) views of zonadhesin immunoreactivity in horse (E. caballus) spermatozoa. C, D) Paired phase contrast and epifluorescence views of zonadhesin immunoreactivity in donkey (E. asinus) spermatozoa. E, F) Same as A–D except zonadhesin in zebra (E. grevyi) spermatozoa. Bars indicate relative magnification of the images.