FIG. 5.

Conservation of zonadhesin D3-polypeptide properties in spermatozoa from three Equus species. Zonadhesin D-polypeptides (protein disulfides reduced) extracted either immediately with 2% SDS/25 mM DTT (Whole) or sequentially with Triton X-100 and SDS were detected by Western blotting as in Figure 1. Detection of the pig D3-polypeptide (p105) is included for comparison. Note the identical sizes and detergent solubilities of the p80 and p110 D3-polypeptides in spermatozoa of all three Equus species.