Figure 5.

Effective inhibition of IFV infection in ex vivo human bronchi by DAS181. Fresh human bronchi sections were infected with A/HK/54/98 (H1N1) and treated with PBS (control) or DAS181 immediately pre-infection (‘pre’), at 24 h post-infection (‘post’) or both (‘pre + post’). Tissues were washed after all DAS181 treatments (2 h at 37°C, 10 µg/cm²). Tissues were incubated for 48 h before homogenization and determination of viral M-gene copy number by qRT–PCR. M-gene copy numbers were normalized to cellular β-actin copy number and are plotted as M-gene copy number per 10⁵ β-actin copy number.