Figure 9.

Sulphated GAG levels produced by differentiating myoblasts isolated from soleus and EDL muscles. Untreated myoblasts (control) or myoblast treated at day 4 with anti-TGFβ1 antibody were cultured as described in Materials and Methods. (a) Total sulphated GAGs at cellular level at the indicated days of culture. Data are mean ± SE from 3 determinations. (b) Sulphated GAG levels in conditioned medium of soleus and EDL derived myoblast cultures. Results were expressed as ng of GAG produced per cell ± SE as a result of 3 determinations. (c) Analysis of GAG species in EDL and Soleus derived myoblasts. HS, expressed as % of total GAG, was determined after a chondroïtinase ABC treatment of the total GAG samples. Two determinations, that did not differ by more than 10%, were used in this experiment.