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RT-PCR analysis and Western blot. The expression of LGI1, BCL2, BAX, and GAPDH was evaluated in HeLa cell clones expressing LGI1 (HeLGI-1, -2, -3) and control cells transfected with empty vector (He-pcDNA3). The results of RT-PCR analysis are shown in panel (a), those of Western blotting in panel (b).
