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. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: Nature. 2009 Jul 13;460(7259):1122–1126. doi: 10.1038/nature08226

Figure 4. Maintained Ebi2 expression impairs participation in GC response.

Figure 4

a, Flow cytometric analysis of spleen cells from an immunized mouse receiving Ebi2-transduced Ig-transgenic (IgMa) B cells, 4 days after transfer, showing gating scheme to identify representation of transduced (hCD4+) cells amongst GC B cells (B220+IgMa+IgDloFashi) and plasma cells (PC, B220loIgMa+). Numbers indicate frequency of cells in the indicated gate. b, Number of transduced (hCD4+) B cells having a GC or plasma cell phenotype. c, Distribution of transduced B cells (hCD4+, blue) in sections of spleen from mice receiving control vector or Ebi2-transduced B cells. Endogenous naïve B cells are stained brown (IgD) and GCs are detected in serial sections using GL7 (blue, lower panels).