Figure 2.

Wild-type and Tsc2^Ek/Ek mutant embryos. (A) Gross appearance of wild-type (left) and mutant (right) E10.5 embryos. Mutant embryo is smaller and has an abnormal head shape. (B) Head of E10.5 mutant embryo exhibiting dysraphia with protruding folds of neuroepithelium indicated by arrow. (C) Sagittal section of the embryo in B; narrow arrow indicates prominent folding of forebrain (F) and wide arrow indicates opening of midbrain (M) to the amniotic cavity. Hindbrain (H) and choroid plexus were morphologically normal.