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In situ hybridization. Sagittal section of E10.5 wild-type embryo showing localization of Tsc2 expression by hybridization of antisense (A) and sense (B) Tsc2–1794 riboprobes to the developing neuroepithelium. F, forebrain; M, midbrain; H, hindbrain. Arrows indicate the neuroepithelium, which was intensely labeled by using antisense Tsc2 riboprobe.
