Table 1.
Tsc2 genotype/phenotype correlation in Long–Evans rats
| Age | Litters, n | No. of embryos
|
|||||
|---|---|---|---|---|---|---|---|
| +/+
|
+/Ek
|
Ek/Ek
|
|||||
| N | A | N | A | N | A | ||
| E18 | 1 | 0 | 0 | 7 | 0 | 0 | 0 (3) |
| E13 | 2 | 3 | 0 | 8 | 0 | 0 | 3 (1) |
| E11 | 3 | 4 | 0 | 21 | 0 (2) | 0 | 5 (3) |
| E10.5 | 1 | 4 | 1 | 5 | 0 | 0 | 2 |
| E9.5 | 4 | 5 | 0 | 14 | 1 (3) | 0 | 2 (3) |
| Total | 11 | 16 | 1 | 55 | 1 (5) | 0 | 12 (10) |
| (17%) | (61%) | (22%) | |||||
N, normal morphology; A, characteristic cranial abnormalities. Parentheses indicate resorbed embryos in these groups. Status of Tsc2 gene was determined by PCR of DNA from Reichert’s membrane or tail tissue of individual embryos.