Table 3.
Clone | Primers | Tm |
---|---|---|
LAX1 | 5'-TTTCTCTCTCCTCCCCAAGAAC-3' | 68 |
LAX2/3 | 5'-CTTTGGATCTGTCATCCAACTTATAG-3' | 71 |
PIN1/10 | 5'-GGCCTCACTTGGTCTCTAGTCTCA-3' | 70 |
PIN4/7 | 5'-GTATTCGAGCCTCATTGGTCTCATC-3' | 70 |
ARR3/4 | 5'-CGAGGGATGTCGGAGTGGAAGAG-3' | 69 |
ARR6 | 5'-CAGCGGGACGAGAGCTCTGGAG-3' | 69 |
Specific primers used in the 3' RACE for the isolation of the 3' part of LAX, PIN, and ARR type A. The annealing temperature is also reported.