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. 2010 Jan 22;5(1):e8781. doi: 10.1371/journal.pone.0008781

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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CaSki cells were infected with serial 10-fold dilutions of HSV-2(G) mixed with each CVL sample. After incubation for 2 h, the inoculum was removed by washing and the cells were overlaid with fresh media. Infection was monitored by counting plaques after 48 h. Results are viral titer (pfu/ml) calculated from 2 independent experiments where each sample was tested in duplicate. The upper panel shows results for each subject for Visit 1 (baseline) versus Visit 4 (post-gel), middle panel for Visit 4 (post-gel) versus Visit 3 (post-gel and postcoital) and lower panel for Visit 2 (postcoital) versus Visit 3 (post-gel and postcoital).