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. 2010 Jan 21;107(6):2461–2466. doi: 10.1073/pnas.0911128107

Fig. 4.

Fig. 4.

Interaction of HCF-1 with Asf1b. (A). Asf1b interacts with either of the HCF-1 two fibronectin repeats (FN-1, FN-1, or FN1 + 2) in two-hybrid analyses. The HCF-1 amino terminal region (HCF-1 aa 360–405) contains one set of sequences that mediate the HCF-1 amino-carboxyterminal subunit association (28). The activation domain vector plasmid (pGADT7) and DNA binding domain-Lamin C fusion were used as negative controls. Control assays are in Fig. S4. (B). Extracts and V5 immunoprecipitates from cells expressing V5-epitope-tagged wild-type HCF-1 (WT) or HCF-1 lacking the fibronectin repeats (ΔFN) and HA-Asf1b were probed for V5 (HCF-1) and HA (Asf1b). Control immunoprecipitates from cells expressing the HCF-1 proteins and UL52 are shown in the Right panel. (C). A schematic of HCF-1 indicating the interactions with Asf1b and the viral replication proteins is shown. The sequences required for HCF-1 amino/carboxyterminal subunit association are indicated (HCF N-C).