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. 2010 Jan 22;6(1):e1000736. doi: 10.1371/journal.ppat.1000736

Figure 3. Protease-sensitive synthetic prions are serially transmissible in Tg9949 mice.

Figure 3

MoSP2-1T, MoSP3-1T and MoSP4-1T were serially passaged to new groups of Tg9949 mice for a second transmission (2T) by intracerebral inoculation of brain homogenate containing each isolate. MoSP2-2T was serially passaged an additional time for a third transmission (3T). In each case, the animals lived a normal life span (Table S4). The brains of Tg9949 mice containing MoSP2-2T, MoSP3-2T, or MoSP4-2T showed no protease-resistant PrP by Western blotting (A), but activity in the ASA (B) and neuropathology consistent with prion disease (C). Brain samples from mice inoculated either with MoSP1 [22] or with homogenates of uninfected Tg9949 mice are shown as controls. (C) Cerebellar sections were stained with H&E (top row) and α-PrP (bottom row). m, molecular layer; gc, granule cell layer. Each scale bar represents 100 µm and applies to the panels in the same row.