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. 2010 Jan 22;6(1):e1000820. doi: 10.1371/journal.pgen.1000820

Figure 4. Effects of mec-12/α-tubulin[K40Q] and cytoplasmic requirement of ELPC-3.

Figure 4

(A) mig-2(gm38) or mig-2gm38);mec-12(e1607) animals were injected with 3ng/µl of the gfp::mec-12[K40] construct. The transgene suppresses the movement phenotypes of both genetic backgrounds. 4 different lines with increasing gfp signal were tested. (B) Expression gfp::mec-12[K40] suppresses the defects in commissures of mig-2(gm38). (C) Expression of NES::elpc-3 (nuclear export signal) but not NLS::elpc-3 (nuclear localization signal) rescues the suppression in mig-2(gm38);elpc-3(tm3120) double mutants. (D) Leptomycin treatment of worms with different genetic backgrounds. Leptomycin A and B suppress the movement defects of mig-2(gm38). The suppression is dependent on mec-12, since mig-2(gm38);mec-12(u76) cannot be suppressed. Rescue of the suppression in the mig-2(gm38); elpc-3(tm3120) EX [elpc-3::gfp], is abolished at high concentrations of Leptomycin A. P values: ns = not significant; *<0,05; **<0,01; ***<0,001. Error bars: SEM.